RNA-binding protein - Quiz
RNA binding protein
  • 1. What does the abbreviation RBP stand for?
A) Ribonucleoprotein binding protein
B) Ribosome-binding protein
C) RNA-binding protein
D) RNA-binding peptide
  • 2. Where are most RBPs located within the cell?
A) Only in the nucleus
B) Only in the mitochondria
C) Only in the cytoplasm
D) Cytoplasmic and nuclear
  • 3. What are heterogeneous ribonucleoprotein particles (hnRNPs)?
A) Complexes of protein and mature mRNA
B) Proteins that bind to DNA
C) Complexes of protein and pre-mRNA in the nucleus
D) Proteins that bind to lipids
  • 4. Which of the following is a structural motif found in RBPs?
A) DNA recognition motif
B) Protein recognition motif
C) Lipid recognition motif
D) RNA recognition motif (RRM)
  • 5. What is a major role of RBPs in post-transcriptional control?
A) Translation
B) Transcription
C) Replication
D) Splicing
  • 6. How many genes encoding RBPs are there in humans according to EuRBPDB?
A) 5000
B) 1500
C) 10000
D) 2961
  • 7. What is the role of RBPs in RNA metabolism?
A) Only regulate RNA transport
B) Regulate every aspect including biogenesis, maturation, transport, localization, and stability
C) Only regulate RNA biogenesis
D) Only regulate RNA stability
  • 8. What type of RNAs do RBPs bind to besides mRNA?
A) Only proteins
B) Only DNA
C) Only mRNA
D) Functional non-coding RNAs
  • 9. Which of the following is a type of non-coding RNA that functions as a ribonucleoprotein complex?
A) MicroRNAs
B) tRNA
C) rRNA
D) mRNA
  • 10. What is the significance of the diversity of RBPs in eukaryotic cells?
A) Allows utilization of RNA exons in various arrangements
B) Reduces the number of introns
C) Limits RNA exons to a single arrangement
D) Prevents RNA exons from being utilized
  • 11. How do RBPs recognize specific RNAs?
A) Through rearrangement of DNA sequences
B) Through rearrangement of basic domains
C) Through rearrangement of lipid sequences
D) Through rearrangement of protein sequences
  • 12. What is the role of RNA-binding proteins in gene expression?
A) Transcriptional regulation
B) Translation regulation
C) Replication regulation
D) Post-transcriptional regulation
  • 13. Which of the following is a function of RBPs in post-transcriptional control?
A) DNA stabilization
B) Lipid stabilization
C) mRNA stabilization
D) Protein stabilization
  • 14. What is the role of RBPs in mRNA localization?
A) They degrade mRNA
B) They inhibit mRNA localization
C) They have no role
D) They play a major role
  • 15. What is the relationship between the diversity of RBPs and the number of introns?
A) Diversity of RBPs is unrelated to the number of introns
B) Diversity of RBPs decreased with the increase in the number of introns
C) Diversity of RBPs increased with the increase in the number of introns
D) Diversity of RBPs prevents the increase in the number of introns
  • 16. What is the role of RBPs in the formation of ribonucleoprotein complexes?
A) They bind to DNA to form these complexes
B) They bind to lipids to form these complexes
C) They bind to proteins to form these complexes
D) They bind to RNA to form these complexes
  • 17. Which of the following is a type of small nuclear RNA?
A) mRNA
B) rRNA
C) Spliceosomal small nuclear RNAs (snRNA)
D) tRNA
  • 18. What is the role of RBPs in RNA biogenesis?
A) They regulate RNA biogenesis
B) They inhibit RNA biogenesis
C) They have no role in RNA biogenesis
D) They degrade RNA during biogenesis
  • 19. What is the role of RBPs in RNA maturation?
A) They regulate RNA maturation
B) They inhibit RNA maturation
C) They have no role in RNA maturation
D) They degrade RNA during maturation
  • 20. Which RNA-binding protein is known for controlling alternative splicing by recognizing specific sequences?
A) U2AF snRNP
B) NOVA1
C) U1 snRNP
D) ADAR
  • 21. What sequence does NOVA1 recognize in RNA for alternative splicing?
A) GCGC
B) UUUU
C) YCAY where Y indicates pyrimidine (U or C).
D) AUGG
  • 22. Where does polyadenylation typically occur in relation to the AAUAAA sequence?
A) About 20 bases downstream.
B) At the 5' untranslated region.
C) About 50 bases upstream.
D) Directly at the AAUAAA sequence.
  • 23. Which protein complex is necessary for the polyadenylation process?
A) Aly/REF.
B) CPSF.
C) TAP/NXF1:p15.
D) FMRP.
  • 24. Which protein is stimulated by CPSF and poly(A)-binding protein to function properly?
A) TAP/NXF1:p15.
B) CPSF.
C) Aly/REF.
D) Poly(A) polymerase.
  • 25. What is the key player in mRNA export?
A) TAP/NXF1:p15 heterodimer.
B) CPSF.
C) ZBP1.
D) Poly(A) polymerase.
  • 26. Which protein recruits TAP by interacting with mRNA?
A) Poly(A) polymerase.
B) CPSF.
C) Aly/REF.
D) ZBP1.
  • 27. Which RBP binds to beta-actin mRNA and localizes it to the lamella region?
A) CPSF.
B) Aly/REF.
C) ZBP1.
D) FMRP.
  • 28. What role does ZBP1 play in the regulation of beta-actin mRNA?
A) It enhances translation by facilitating ribosome binding.
B) It represses translation by blocking translation initiation.
C) It degrades beta-actin mRNA.
D) It modifies beta-actin mRNA sequences.
  • 29. When do some RNA-binding proteins remain bound to RNA?
A) Only during translation.
B) From transcription until degradation.
C) Only during DNA replication.
D) Only during RNA splicing.
  • 30. Which method is used to identify direct RNA binding sites of RNA-binding proteins?
A) Cross-linking immunoprecipitation (CLIP).
B) Gel electrophoresis.
C) Polymerase chain reaction (PCR).
D) Southern blotting.
  • 31. How many amino acids typically make up the RNA-recognition motif (RRM)?
A) 100–110 amino acids
B) 75–85 amino acids
C) 50–60 amino acids
D) 30–40 amino acids
  • 32. What structural elements form the RNA-recognition motif (RRM)?
A) Three α-helices and a two-stranded β-sheet
B) A single α-helix and a five-stranded β-sheet
C) A four-stranded β-sheet against two α-helices
D) Two α-helices against a three-stranded β-sheet
  • 33. How many structures of the RRM have been identified through NMR spectroscopy and X-ray crystallography?
A) Ten
B) Twenty
C) Fifteen
D) Five
  • 34. What is the size range of the double-stranded RNA-binding motif (dsRM)?
A) 70–75 amino acids
B) 100–110 amino acids
C) 30–40 amino acids
D) 50–60 amino acids
  • 35. Which RNA-binding protein is involved in regulating tissue-specific alternative splicing during somatic development?
A) Sam68
B) CIRBP
C) ZBP1
D) MEC-8
  • 36. Which RNA-binding protein specifically binds to the UUGUUGUGUUGU mRNA stretch?
A) Slr1
B) CIRBP
C) Sam68
D) UNC-75
  • 37. In which cells does UNC-75 activate splicing between exon 7a and exon 8?
A) Hypodermis cells
B) Candida albicans cells
C) Excretory canal cells
D) Neuronal cells
  • 38. Which RNA-binding protein is involved in controlling polarized growth in Candida albicans?
A) Sam68
B) UNC-75
C) CIRBP
D) Slr1
  • 39. Which RNA-binding protein is associated with fragile X-associated tremor/ataxia syndrome when lost?
A) Sam68
B) Slr1
C) CIRBP
D) UNC-75
  • 40. Which RNA-binding protein is involved in controlling the cellular response to hypoxia?
A) UNC-75
B) CIRBP
C) Sam68
D) Slr1
  • 41. Which RNA-binding protein is differentially expressed in lung cancer due to CNV losses?
A) UNC-75
B) Sam68
C) CIRBP
D) KHDRBS2
Created with That Quiz — where test making and test taking are made easy for math and other subject areas.