Growth medium - Quiz
Growth medium
  • 1. What is a minimal medium defined as?
A) A defined medium with just enough ingredients to support growth
B) A complex medium used for human cells
C) A medium that forbids all bacterial growth
D) A medium containing only amino acids
  • 2. What is typically omitted from minimal media?
A) Amino acids
B) Water
C) Salts
D) Carbon sources
  • 3. What type of microorganisms are often grown using minimal media?
A) Only Gram-negative bacteria
B) Wild-type
C) Pathogenic viruses
D) Mutated animal cells
  • 4. What is an example of a carbon source in minimal media?
A) Glucose
B) Tetracycline
C) Neomycin
D) Ampicillin
  • 5. What do the salts in minimal media provide?
A) Glucose and sucrose
B) Magnesium, nitrogen, phosphorus, and sulfur
C) Antibiotics and dyes
D) Proline and histidine
  • 6. What are supplementary minimal media used for?
A) Culturing specific lines of auxotrophic recombinants
B) Killing all bacteria in a sample
C) Growing wild-type microorganisms exclusively
D) Replacing water in the growth process
  • 7. What is the purpose of selective media?
A) Preventing all bacterial metabolism
B) Growth of only selected microorganisms
C) Measuring the concentration of water
D) Growing all types of bacteria equally
  • 8. Which antibiotics are mentioned as agents used to create selective media?
A) Nalidixic acid and colistin
B) Penicillin and aspirin
C) Gancyclovir and neomycin
D) Ampicillin or tetracycline
  • 9. What term is used for a gene that allows a cell to grow in a selective medium?
A) Auxotroph
B) Exconjugant
C) Marker
D) Recombinant
  • 10. What is used in selective media for eukaryotic cells to select transfected cells?
A) Proline
B) Histidine
C) Neomycin
D) Glucose
  • 11. What is the exception to the rule that markers allow cell growth in selective media?
A) Gancyclovir
B) Tetracycline
C) Neomycin
D) Ampicillin
  • 12. Which substance in Eosin methylene blue makes it toxic for Gram-positive bacteria?
A) Salt
B) Agar
C) Dyes
D) Glucose
  • 13. What characteristic of YM and MEA agar deters bacterial growth?
A) High salt concentration
B) Low pH
C) Lack of water
D) High antibiotic content
  • 14. What is MacConkey agar used for?
A) Virus isolation
B) Gram-positive bacteria
C) Gram-negative bacteria
D) Fungi only
  • 15. What does HIS-selective medium lack?
A) Histidine
B) Glucose
C) Proline
D) Salts
  • 16. What is Mannitol salt agar differential for?
A) Gram-negative bacteria
B) Legionella
C) Mannitol
D) Staphylococci
  • 17. Which medium is selective for Legionella pneumophila?
A) Baird-Parker agar
B) MacConkey agar
C) Sabouraud agar
D) Buffered charcoal yeast extract agar
  • 18. What is the primary use of Baird-Parker agar?
A) Ectomycorrhizal fungi
B) Legionella pneumophila
C) Gram-positive staphylococci
D) Moulds and yeasts
  • 19. What contributes to Sabouraud agar's selectivity for fungi?
A) High salt concentration
B) Presence of antibiotics only
C) Low pH and high glucose
D) Lack of nitrogen
  • 20. What is the purpose of dichloran and rose Bengal in DRBC agar?
A) To kill all yeast cells
B) To restrict the growth of mould colonies
C) To provide nitrogen to the fungi
D) To increase the pH of the medium
  • 21. Which media are used for ectomycorrhizal fungi?
A) Baird-Parker and DRBC
B) MMN and BAF medium
C) MacConkey and Hektoen
D) CNA and Sabouraud
  • 22. What is the function of nalidixic acid and colistin in CNA agar?
A) To act as a carbon source
B) To inhibit Gram-negative organisms
C) To promote the growth of mould
D) To kill all Gram-positive bacteria
  • 23. What is a defining characteristic of a chemically defined medium?
A) The exact composition is unknown
B) It contains animal tissue
C) All chemicals used are known
D) It contains yeast extract
  • 24. Which ingredient is strictly absent from a defined medium?
A) Salts
B) Plant tissue
C) Water
D) Glucose
  • 25. What substance is required in an undefined medium alongside salts and carbon sources?
A) Plant hormones
B) Animal blood
C) Antibiotics
D) Water
  • 26. Where are bacteria typically kept in long-term laboratory settings using general culture media?
A) Defined synthetic environments
B) Sterile air chambers
C) Laboratory culture collections
D) Natural soil samples
  • 27. What is a growth medium designed to support?
A) Cell proliferation
B) Cell desiccation
C) Cell apoptosis
D) Cell mutation
  • 28. Which plant is specifically mentioned as being grown in growth media?
A) Physcomitrella patens
B) Zea mays
C) Oryza sativa
D) Arabidopsis thaliana
  • 29. What type of organisms are viruses?
A) Obligate intracellular parasites
B) Facultative anaerobes
C) Autotrophic microbes
D) Extracellular saprophytes
  • 30. Which bacterial genus is capable of decomposing agar?
A) Streptococcus
B) Escherichia
C) Staphylococcus
D) Bacillus
  • 31. What do bacteria grown in liquid cultures often form?
A) Sedimentary layers
B) Colloidal suspensions
C) Biofilm mats
D) Crystalline structures
  • 32. What must be added to animal cell media to support their growth?
A) Blood serum
B) Agarose
C) Glucose only
D) Ammonium salts
  • 33. What is the liquid used to make beer that serves as a growth medium for yeast?
A) Tincture
B) Must
C) Mash
D) Wort
  • 34. What is produced when yeast grows in wort under anaerobic conditions?
A) Acetic acid
B) Hydrogen gas
C) Alcohol
D) Lactic acid
  • 35. What term describes organisms that require specialized environments due to complex needs?
A) Opportunistic
B) Fastidious
C) Ubiquitous
D) Autotrophic
  • 36. What is the primary function of differential media?
A) Preventing microbial multiplication
B) Supporting fastidious organisms only
C) Distinguishing one microorganism type from another
D) Storing specimens for long periods
  • 37. Which of the following is an example of an indicator used in differential media?
A) Glycerol
B) Sodium chloride
C) Charcoal
D) Neutral red
  • 38. What does blood agar become in the presence of beta-hemolytic organisms?
A) Brown
B) Opaque
C) Transparent
D) Red
  • 39. What color do colonies of Streptococcus agalactiae appear on Granada medium?
A) Red
B) Brown
C) Blue
D) Transparent
  • 40. X-gal plates are used to differentiate what?
A) Gonococci
B) Lac operon mutants
C) Enteric bacilli
D) Strict anaerobes
  • 41. Transport media for anaerobes must be free of what?
A) Lactose
B) Glycerol
C) Charcoal
D) Molecular oxygen
  • 42. What do enriched media contain to support fastidious organisms?
A) Bacterial inhibitors
B) Extra nutrients
C) Carbon-free buffers
D) Reducing agents
  • 43. At what temperature range is blood treated to create chocolate agar?
A) 80–85 °C
B) 60–65 °C
C) 20–25 °C
D) 40–45 °C
  • 44. Eosin methylene blue is differential for what?
A) Anaerobic growth
B) Beta-hemolysis
C) Mannitol fermentation
D) Lactose fermentation
  • 45. Thioglycolate broth is designed for which type of organisms?
A) Strict anaerobes
B) Lac operon mutants
C) Enteric bacilli
D) Fastidious aerobes
  • 46. What should transport media maintain in specimens?
A) Active fermentation
B) Viability
C) High concentration
D) Increased metabolic rate
  • 47. What is a potential risk associated with using serum-containing media?
A) Lack of growth factors
B) Inability to support cell growth
C) Insufficient glucose levels
D) Contamination issues
  • 48. Which of the following media types is known for having a standardized formulation?
A) Animal-derived medium
B) Serum-free media
C) Fetal bovine serum
D) Serum-containing media
  • 49. Why is the choice of media important for protein expression?
A) It prevents the production of proteins
B) It determines the genetic sequence of the protein
C) It influences therapeutic characteristics like glycosylation
D) It eliminates the need for purification
  • 50. What are Plasmax and HPLM examples of?
A) Media used only for bacterial cultures
B) Serum-containing growth media
C) Media that represent physiological nutrient levels
D) Chemically undefined media
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