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Growth medium - Quiz
Contributed by: Pope
  • 1. What is a minimal medium defined as?
A) A defined medium with just enough ingredients to support growth
B) A complex medium used for human cells
C) A medium that forbids all bacterial growth
D) A medium containing only amino acids
  • 2. What is typically omitted from minimal media?
A) Carbon sources
B) Water
C) Salts
D) Amino acids
  • 3. What type of microorganisms are often grown using minimal media?
A) Wild-type
B) Pathogenic viruses
C) Only Gram-negative bacteria
D) Mutated animal cells
  • 4. What is an example of a carbon source in minimal media?
A) Glucose
B) Ampicillin
C) Neomycin
D) Tetracycline
  • 5. What do the salts in minimal media provide?
A) Antibiotics and dyes
B) Proline and histidine
C) Glucose and sucrose
D) Magnesium, nitrogen, phosphorus, and sulfur
  • 6. What are supplementary minimal media used for?
A) Growing wild-type microorganisms exclusively
B) Killing all bacteria in a sample
C) Culturing specific lines of auxotrophic recombinants
D) Replacing water in the growth process
  • 7. What is the purpose of selective media?
A) Preventing all bacterial metabolism
B) Growing all types of bacteria equally
C) Measuring the concentration of water
D) Growth of only selected microorganisms
  • 8. Which antibiotics are mentioned as agents used to create selective media?
A) Penicillin and aspirin
B) Ampicillin or tetracycline
C) Gancyclovir and neomycin
D) Nalidixic acid and colistin
  • 9. What term is used for a gene that allows a cell to grow in a selective medium?
A) Exconjugant
B) Recombinant
C) Auxotroph
D) Marker
  • 10. What is used in selective media for eukaryotic cells to select transfected cells?
A) Glucose
B) Neomycin
C) Histidine
D) Proline
  • 11. What is the exception to the rule that markers allow cell growth in selective media?
A) Gancyclovir
B) Neomycin
C) Tetracycline
D) Ampicillin
  • 12. Which substance in Eosin methylene blue makes it toxic for Gram-positive bacteria?
A) Dyes
B) Salt
C) Glucose
D) Agar
  • 13. What characteristic of YM and MEA agar deters bacterial growth?
A) Lack of water
B) High salt concentration
C) High antibiotic content
D) Low pH
  • 14. What is MacConkey agar used for?
A) Fungi only
B) Gram-positive bacteria
C) Gram-negative bacteria
D) Virus isolation
  • 15. What does HIS-selective medium lack?
A) Histidine
B) Glucose
C) Proline
D) Salts
  • 16. What is Mannitol salt agar differential for?
A) Legionella
B) Gram-negative bacteria
C) Staphylococci
D) Mannitol
  • 17. Which medium is selective for Legionella pneumophila?
A) Buffered charcoal yeast extract agar
B) Sabouraud agar
C) MacConkey agar
D) Baird-Parker agar
  • 18. What is the primary use of Baird-Parker agar?
A) Gram-positive staphylococci
B) Legionella pneumophila
C) Ectomycorrhizal fungi
D) Moulds and yeasts
  • 19. What contributes to Sabouraud agar's selectivity for fungi?
A) Lack of nitrogen
B) High salt concentration
C) Low pH and high glucose
D) Presence of antibiotics only
  • 20. What is the purpose of dichloran and rose Bengal in DRBC agar?
A) To restrict the growth of mould colonies
B) To provide nitrogen to the fungi
C) To kill all yeast cells
D) To increase the pH of the medium
  • 21. Which media are used for ectomycorrhizal fungi?
A) MMN and BAF medium
B) Baird-Parker and DRBC
C) CNA and Sabouraud
D) MacConkey and Hektoen
  • 22. What is the function of nalidixic acid and colistin in CNA agar?
A) To kill all Gram-positive bacteria
B) To act as a carbon source
C) To inhibit Gram-negative organisms
D) To promote the growth of mould
  • 23. What is a defining characteristic of a chemically defined medium?
A) It contains yeast extract
B) All chemicals used are known
C) It contains animal tissue
D) The exact composition is unknown
  • 24. Which ingredient is strictly absent from a defined medium?
A) Salts
B) Plant tissue
C) Glucose
D) Water
  • 25. What substance is required in an undefined medium alongside salts and carbon sources?
A) Animal blood
B) Water
C) Antibiotics
D) Plant hormones
  • 26. Where are bacteria typically kept in long-term laboratory settings using general culture media?
A) Sterile air chambers
B) Natural soil samples
C) Laboratory culture collections
D) Defined synthetic environments
  • 27. What is a growth medium designed to support?
A) Cell apoptosis
B) Cell proliferation
C) Cell desiccation
D) Cell mutation
  • 28. Which plant is specifically mentioned as being grown in growth media?
A) Zea mays
B) Physcomitrella patens
C) Arabidopsis thaliana
D) Oryza sativa
  • 29. What type of organisms are viruses?
A) Autotrophic microbes
B) Obligate intracellular parasites
C) Facultative anaerobes
D) Extracellular saprophytes
  • 30. Which bacterial genus is capable of decomposing agar?
A) Staphylococcus
B) Escherichia
C) Streptococcus
D) Bacillus
  • 31. What do bacteria grown in liquid cultures often form?
A) Colloidal suspensions
B) Sedimentary layers
C) Crystalline structures
D) Biofilm mats
  • 32. What must be added to animal cell media to support their growth?
A) Agarose
B) Glucose only
C) Blood serum
D) Ammonium salts
  • 33. What is the liquid used to make beer that serves as a growth medium for yeast?
A) Must
B) Mash
C) Wort
D) Tincture
  • 34. What is produced when yeast grows in wort under anaerobic conditions?
A) Lactic acid
B) Hydrogen gas
C) Alcohol
D) Acetic acid
  • 35. What term describes organisms that require specialized environments due to complex needs?
A) Ubiquitous
B) Fastidious
C) Autotrophic
D) Opportunistic
  • 36. What is the primary function of differential media?
A) Storing specimens for long periods
B) Preventing microbial multiplication
C) Distinguishing one microorganism type from another
D) Supporting fastidious organisms only
  • 37. Which of the following is an example of an indicator used in differential media?
A) Sodium chloride
B) Neutral red
C) Glycerol
D) Charcoal
  • 38. What does blood agar become in the presence of beta-hemolytic organisms?
A) Opaque
B) Red
C) Transparent
D) Brown
  • 39. What color do colonies of Streptococcus agalactiae appear on Granada medium?
A) Red
B) Transparent
C) Blue
D) Brown
  • 40. X-gal plates are used to differentiate what?
A) Enteric bacilli
B) Strict anaerobes
C) Gonococci
D) Lac operon mutants
  • 41. Transport media for anaerobes must be free of what?
A) Lactose
B) Glycerol
C) Molecular oxygen
D) Charcoal
  • 42. What do enriched media contain to support fastidious organisms?
A) Bacterial inhibitors
B) Carbon-free buffers
C) Reducing agents
D) Extra nutrients
  • 43. At what temperature range is blood treated to create chocolate agar?
A) 20–25 °C
B) 40–45 °C
C) 60–65 °C
D) 80–85 °C
  • 44. Eosin methylene blue is differential for what?
A) Mannitol fermentation
B) Lactose fermentation
C) Beta-hemolysis
D) Anaerobic growth
  • 45. Thioglycolate broth is designed for which type of organisms?
A) Fastidious aerobes
B) Enteric bacilli
C) Strict anaerobes
D) Lac operon mutants
  • 46. What should transport media maintain in specimens?
A) Increased metabolic rate
B) Active fermentation
C) Viability
D) High concentration
  • 47. What is a potential risk associated with using serum-containing media?
A) Contamination issues
B) Inability to support cell growth
C) Insufficient glucose levels
D) Lack of growth factors
  • 48. Which of the following media types is known for having a standardized formulation?
A) Serum-free media
B) Fetal bovine serum
C) Animal-derived medium
D) Serum-containing media
  • 49. Why is the choice of media important for protein expression?
A) It prevents the production of proteins
B) It determines the genetic sequence of the protein
C) It eliminates the need for purification
D) It influences therapeutic characteristics like glycosylation
  • 50. What are Plasmax and HPLM examples of?
A) Media used only for bacterial cultures
B) Chemically undefined media
C) Serum-containing growth media
D) Media that represent physiological nutrient levels
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